在新药研发、药物作用机制解析两大核心科研领域如何从海量蛋白质样本中快速锁定药物直接作用靶标是长期存在的核心技术挑战。生命科学进入系统生物学发展阶段后传统单一靶点验证实验模式局限性凸显无法完整解析药物多重药效对应的分子作用通路。HuProt™20K human proteome microarray20K 人类蛋白组芯片拥有高通量、高基因组覆盖、蛋白直接结合检测三大核心技术优势为药物靶点筛选搭建标准化实验体系辅助科研人员优化蛋白层面科研探索流程。这款芯片的核心技术载体为全覆盖体外人源重组蛋白文库。芯片表面独立固定超 21000 种全长人源重组蛋白覆盖人类基因组约 81% 开放阅读框包含激酶、细胞膜受体、转录因子、细胞骨架蛋白等三十余种功能蛋白。为保证实验数据贴合人体真实生理状态全部蛋白均采用酵母真核表达系统单独制备搭配 N 端 GST-His6 双标签纯化工艺定向富集折叠结构正常的活性蛋白解决原核表达系统产出蛋白丧失生物活性的行业常见问题。针对小分子单体化合物靶点筛选工作HuProt™第四代人类蛋白组芯片具备独特的科研增值效应。凭借超高蛋白覆盖广度单次实验采集的数据不属于一次性实验材料而是可反复挖掘、长期参考的靶点图谱。仅针对单一小分子药物开展检测就能一次性检出几十至上百个潜在直接互作蛋白国内大量科研团队依托该技术实现单次筛选产出多项学术成果。重庆医科大学课题组依托同一套芯片筛选原始数据分年度发表多篇高水平学术文献分别揭示淫羊藿苷靶向 PRDX3 调控视网膜小胶质细胞功能、靶向 HK2 减轻视网膜病变损伤的两套独立分子机制。成都中医药大学研究团队持续使用该芯片开展机制研究产出多项科研成果完整梳理芍药苷调控胃黏膜修复、缓解胆汁淤积、干预溃疡性结肠炎的多靶点调控逻辑。这种一测多用的科研范式直观体现 20K 人类蛋白组芯片在压缩科研周期、提升产出效率上的突出价值。总体来看Human Microarray Proteomic Analysis 提供高通量蛋白直接互作检测数据帮助科研人员在课题启动阶段筛选可信度更高的候选药物靶标大幅缩减实验试错成本。不管是活性明确但作用机制空白的新药初筛项目还是需要系统拆解多靶点药物调控网络的深度机制课题该芯片技术都能提供强有力、稳定的科研技术支撑。达吉特20K人类蛋白组芯片靶点筛选实验客户服务案例Chen YJ, Shi RC, Xiang YC, et al. Malate initiates a proton-sensing pathway essential for pH regulation of inflammation.Signal Transduct Target Ther.2024;9(1):367. Published 2024 Dec 30. doi:10.1038/s41392-024-02076-9(陆军军医大学附属第一医院)IF40.8Hu M, Xu Y, Wang Y, et al. Gut microbial-derived N-acetylmuramic acid alleviates colorectal cancer via the AKT1 pathway.Gut.Published online February 27, 2025. doi:10.1136/gutjnl-2024-332891(南方医科大学珠江医院)IF23.1Shao M, Zhang X, Sun J, et al. Saikosaponin b1 Attenuates Liver Fibrosis by Blocking STAT3/Gli1 Interaction and Inducing Gli1 Degradation.Exploration.2766-8509.(浙江中医药大学)IF22.5Xu Q, Li G, Zhang H, et al. The natural flavonoid dihydromyricetin targets senescent cells via PRDX2 and alleviates age-related diseases.Nat Commun.Published online March 6, 2026. doi:10.1038/s41467-026-70302-9(上海交通大学医学院)IF15.7Chen L, Wu B, Mo L, et al. High-content screening identifies ganoderic acid A as a senotherapeutic to prevent cellular senescence and extend healthspan in preclinical models.Nat Commun.2025;16(1):2878. Published 2025 Mar 24. doi:10.1038/s41467-025-58188-5(华中科技大学同济医学院)IF15.7Gu Y, Chen Y, Wei L, et al. Author Correction: ABHD5 inhibits YAP-induced c-Met overexpression and colon cancer cell stemness via suppressing YAP methylation.Nat Commun.2023;14(1):1875. Published 2023 Apr 4. doi:10.1038/s41467-023-37521-w(第三军医大学) IF15.7Zhong J, He X, Gao X, et al. Hyodeoxycholic acid ameliorates nonalcoholic fatty liver disease by inhibiting RAN-mediated PPARα nucleus-cytoplasm shuttling.Nat Commun.2023;14(1):5451. Published 2023 Sep 6. doi:10.1038/s41467-023-41061-8(上海中医药大学)IF15.7Shen SM, Ji Y, Zhang C, et al. Nuclear PTEN safeguards pre-mRNA splicing to link Golgi apparatus for its tumor suppressive role.Nat Commun.2018;9(1):2392. Published 2018 Jun 19. doi:10.1038/s41467-018-04760-1(上海交通大学)IF15.7Li D, Wei R, Zhang X, et al. Gut commensal metabolite rhamnose promotes macrophages phagocytosis by activating SLC12A4 and protects against sepsis in mice.Acta Pharm Sin B.2024;14(7):3068-3085. doi:10.1016/j.apsb.2024.03.025(南方医科大学) IF14.6Xu X, Yan Y, Zheng M, et al. Emodin Alleviates Sepsis-Induced Multiorgan Damage by Inhibiting NETosis through Targeting Neutrophils BCL-10.Adv Sci (Weinh).2025 Aug 8:e17129.(首都医科大学附属北京中医医院)IF14.1Xuan C, Chen D, Zhang S, et al. Isoquercitrin Alleviates Diabetic Nephropathy by Inhibiting STAT3 Phosphorylation and Dimerization.Adv Sci (Weinh).Published online April 4, 2025. doi:10.1002/advs.202414587(中国人民解放军总医院) IF14.1Cui J, Chen Y, Yang Q, et al. Protosappanin A Protects DOX-Induced Myocardial Injury and Cardiac Dysfunction by Targeting ACSL4/FTH1 Axis-Dependent Ferroptosis.Adv Sci (Weinh).2024;11(34):e2310227. doi:10.1002/advs.202310227(哈尔滨医科大学附属第二医院)IF14.1Zheng Y, Zhao L, Xiong Z, et al. Ursolic acid targets secreted phosphoprotein 1 to regulate Th17 cells against metabolic dysfunction-associated steatotic liver disease.Clin Mol Hepatol.2024;30(3):449-467. doi:10.3350/cmh.2024.0047(上海中医药大学联合广州中医药大学) IF14Zhou WJ, Yang HL, Mei J, et al. Fructose-1,6-bisphosphate prevents pregnancy loss by inducing decidual COX-2 macrophage differentiation.Sci Adv.2022;8(8):eabj2488. doi:10.1126/sciadv.abj2488(复旦大学附属妇产科医院)IF12.5Zhao T, Zeng J, Xu Y, et al. Chitinase-3 like-protein-1 promotes glioma progression via the NF-κB signaling pathway and tumor microenvironment reprogramming.Theranostics.2022;12(16):6989-7008. Published 2022 Oct 3. doi:10.7150/thno.75069(广州医科大学) IF12.4